January 29, 2011

Oxford Nanopore GridION, Genome Sequencing Servers

Oxford Nanopore’s GridION Genome Sequencing Servers utilize semiconductor-like technology for high throughput Genome sequencing.

Like Life Technologies Ion PGM Sequencer, the GridION provides wells on a chip for the reaction. The chip sits on a one-time usable cartridge which contains the reagents and which plugs into the server (called a node by Nanopore). Each chip contains highly scalable arrays of nanopores for sensing at the single molecule level. Active nanopores can scale from tens to hundreds of thousands per chip. Applications range from DNA sequencing, protein analysis to small molecule analysis.

Nanopore’s approach is unique in providing servers that scale to large numbers that means thousands or millions of genome sequencing experiments could be run concurrently giving very high genome sequencing throughputs. Nanopore is said to be working with Illumina who has their MiSeq, personal sequencer and the everygenome sequencing service.

Nanopore’s GridION servers (each node at 50W) are designed to fit in standard server racks and are networked such that the genome sequencing reactions can be controlled and results viewed in real-time over the Internet. Similar to Amazon EC2 for cloud computing, the GridION will enable cloud genome sequencing and be upstream of cloud sequence analysis services such as DNANexus.

The GridION system: part 1 from Oxford Nanopore on Vimeo.

The GridION system: Part 2, advanced workflows from Oxford Nanopore on Vimeo.

Nanopore sequencing from Oxford Nanopore on Vimeo.