January 19, 2011

See Genome Work in the Cell

If you want to see a Genome work in the cell, here is a technique  (native elongating transcript sequencing, NET-seq) pioneered by UCSF researchers.

UCSF researchers using biochemical techniques, DNA sequencing and computers were able to examine the process of cell converting a DNA into RNA which is used to create protein that govern most biological functions.

By overlaying those maps with their own maps of RNA production, the scientists were able to observe for the first time that polymerase comes in direct contact with the histone proteins during the transcription process, while also seeing how the nucleosomes acted as a speed bump for the polymerase enzyme as it moved along the genome transcribing DNA into RNA. In addition, the research showed that the organization of histone marks controlled whether “junk RNA” was produced from a given region of DNA.