February 2, 2011

Nanopore Sequencing, Genome Sequencing In 24 hours

Nanopore sequencing, can lead to genome sequencing in 24 hours. The promise of Nanopore Sequencing from NIH.

A nanopore-based device provides single-molecule detection and analytical capabilities that are achieved by electrophoretically driving molecules in solution through a nano-scale pore. The nanopore provides a highly confined space within which single nucleic acid polymers can be analyzed at high throughput by one of a variety of means, and the perfect processivity that can be enforced in a narrow pore ensures that the native order of the nucleobases in a polynucleotide is reflected in the sequence of signals that is detected. Kilobase length polymers (single-stranded genomic DNA or RNA) or small molecules (e.g., nucleosides) can be identified and characterized without amplification or labeling, a unique analytical capability that makes inexpensive, rapid DNA sequencing a possibility. Further research and development to overcome current challenges to nanopore identification of each successive nucleotide in a DNA strand offers the prospect of `third generation' instruments that will sequence a diploid mammalian genome for ~$1,000 in ~24 h.

Oxford Nanopore’s GridION is one of the first products to utilize nanopore sequencing. Architected as Genome Sequencing Servers, they are designed for highly scalable, high throughput genome sequencing or molecular analysis. More details at the AGBT conference today.